Cloning, Expression, Sequence Analysis and Homology Modeling of the Prolyl Endoprotease from Eurygaster integriceps Puton
نویسندگان
چکیده
منابع مشابه
Cloning, Expression, Sequence Analysis and Homology Modeling of the Prolyl Endoprotease from Eurygaster integriceps Puton
eurygaster integriceps Puton, commonly known as sunn pest, is a major pest of wheat in Northern Africa, the Middle East and Eastern Europe. This insect injects a prolyl endoprotease into the wheat, destroying the gluten. The purpose of this study was to clone the full length cDNA of the sunn pest prolyl endoprotease (spPEP) for expression in E. coli and to compare the amino acid sequence of the...
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The susceptibility of adult sunn pest, Eurygaster integriceps Puton (Hemiptera: Scutelleridae), to five local Syrian isolates of Heterorhabditis bacteriophora, Steinernema riobravae Texas, S. carpocapsae C3B, and H. bacteriophora 8-14 strains was evaluated under laboratory conditions. Concentrations of 50, 100, 200, and 400 nematodes per adult were bioassayed; for each nematode concentration, f...
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The Sunn pest, Eurygaster integriceps Puton (Heteroptera: Scutelleridae), is one of the most important pests of wheat and causes considerable damage to this valuable crop annually. Digestive proteinase activity of adult insects was investigated using general and specific substrates and inhibitors. Proteolytic activity was low when the common conventional substrates, azoalbumin, azocasein and he...
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ژورنال
عنوان ژورنال: Insects
سال: 2014
ISSN: 2075-4450
DOI: 10.3390/insects5040762